Dr. Eyal Golub

Telephone

+972-3-5318071

eyal.golub@biu.ac.il

Office

building 206, room C262

Fields of Interest

Research categories:

Biochemistry & Biophysics, Macromolecular Structure, Nanotechnology.

Research Categories

Dr. Eyal Golub obtained both his BSc and MSc from Bar-Ilan University, Ramat Gan, Israel. He pursued a PhD position at the Hebrew University, Jerusalem, Israel, under the supervision of Prof. Itamar Willner, where he worked on the development of sensitive sensing platforms and new paradigms for designing DNA-based catalysis. He became an EMBO Postdoctoral Fellow at the University of California, San Diego, working with Prof. Akif Tezcan on metal-directed self-assembly of ordered protein structures.

At 2020 Dr. Golub joined the Chemistry Department at Bar-Ilan University. The Golub Lab will be addressing fundamental aspects of biomolecular interactions and exploring emergent properties within such constructs by taking a creative and interdisciplinary approach, meshing the fields of chemistry, nanotechnology and protein engineering.

Education:

2016-2019 Postdoctoral research scholar, Department of Chemistry and Biochemistry, University of California, San Diego. Advisor: Prof. Akif Tezcan.

2009-2015 PhD in Chemistry, Institute of Chemistry, The Hebrew University of Jerusalem, Israel. Advisor: Prof. Itamar Willner.

2005-2008 MSc in Chemistry, Chemistry Department, Bar Ilan University, Ramat Gan. Advisor: Prof. Arie Zaban.

2002-2005 BSc in Chemistry, Chemistry Department, Bar Ilan University, Ramat Gan.

Position:

2020-present Senior Lecturer, Department of Chemistry, Bar-Ilan University.

2019-2020 Research associate, the department of Material Science and Engineering, Technion.

Research

Our passion at the Golub lab is to design and explore through chemical and biological means how the assembly of biomolecules bring about the structural and functional diversity that are present in all life forms. While individual protein and DNA molecules exhibit an impressive set of properties their assemblies are by far more elaborate and sophisticated since they can create unique microenvironments and interfaces. However, pinning down or predicting the origins for any synergistic interactions is challenging due to the complexity of the constructs and the inability to discern between identical subcomponents. Motivated by this challenge our group aims to develop new methods and approaches for the controlled assembly of biomolecules that exhibit novel characteristics.

On the same time, we aim to expand the scope of known protein-DNA interactions while focusing on non-canonical G-quadruplex DNA structures. Although these structures are abundant in nature and associated with fundamental biological processes, the biomolecular interactions that control their affinity and specificity towards proteins are still largely underexplored. Our approach is two-fold: elucidating new structures of protein-G-quadruplex complexes on the one hand and exploring the protein the molecular basis for G-quadruplex recognition.

To these ends we will use an interdisciplinary approach drawing inspiration and expertise both from the world of chemistry and synthetic biology. Among these will be a broad range of tools including chemical synthesis, molecular biology, spectroscopy, single crystal X-ray crystallography and other techniques to characterize biophysical and photophysical properties.

Publications

Articles:

E. Golub, G. Pelossof, R. Freeman, H. Zhang and I. Willner, Electrochemical, Photoelectrochemical and Surface Plasmon Resonance Detection of Cocaine Using Supramolecular Aptamer Complexes and Metallic or Semiconductor Nanoparticles, Anal. Chem., 81, 9291-9298 (2009).
E. Golub, R. Freeman and I. Willner The Hemin/G-Quadruplex Acts as NADH Oxidase and NADH Peroxidase Mimicking DNAzyme. Angew. Chem. Int. Ed., 50, 11710-11714 (2011).
E. Golub, R. Freeman, A. Niazov and I. Willner Hemin/G-Quadruplexes as DNAzymes for the Fluorescent Detection of DNA, Aptamer-Thrombin Complexes and Probing the Activity of Glucose Oxidase. Analyst, 136, 4397-4401 (2011).
X. Liu, R. Freeman, E. Golub and I. Willner Chemiluminescence and Chemiluminescence Resonance Energy Transfer (CRET) Aptamer Sensors Using Catalytic Hemin/G-Quadruplexes. ACS Nano, 5, 7648-7655 (2011).
E. Golub, A. Niazov, R. Freeman, M. Zatsepin and I. Willner Photoelectrochemical Biosensors Without an External Irradiation: Probing Enzyme Activities and DNA Sensing Using Hemin/G-Quadruplex-Stimulated Chemiluminescence Resonance Energy Transfer (CRET) Generation of Photocurrents. J. Phys. Chem. C, 116, 13827-13834 (2012).
E. Golub, R. Freeman and I. Willner, Hemin/G-Quadruplex-Catalyzed Aerobic Oxidation of Thiols to Disulfides: Application of the Process for the Development of Sensors, Aptasensors, and for Probing Acetylcholine Esterase Activity, Anal. Chem., 85, 12126-12133 (2013).
E. Sharon, E. Golub, A. Niazov-Elkan, D. Balogh and I Willner Analysis of Telomerase by the Telomeric/G-Quadruplex-Controlled Aggregation of Au NPs in the Presence of Cysteine. Anal. Chem., 86, 3153-3158 (2014).
A. Niazov-Elkan, E. Golub, E. Sharon, D. Balogh, and I. Willner, DNA Sensors and Aptasensors Based on the Hemin/G-quadruplex-Controlled Aggregation of Au NPs in the Presence of L-Cysteine, Small, 10, 2883-2891 (2014).
Y. Hu, F. Wang, C.-H. Lu, J. Girsh, E. Golub and I. Willner Switchable Enzyme/DNAzyme Cascades by the Reconfiguration of DNA Nanostructures, Chem. Eur. J., 20, 16203-16209 (2014), link.
N. Enkin, E. Sharon, E. Golub and I Willner Ag Nanocluster/DNA Hybrids: Functional Modules for the Detection of Nitroaromatic and RDX Explosives, Nano Lett., 14, 4918-4922 (2014).
H. B. Albada, E. Golub and I. Willner Computational docking simulations of a DNA-aptamer for argininamide and related ligands, J. Comput. Aided Mol. Des., 29, 643-654 (2015).
L. Freage, A. Trifonov, R. Tel-Vered, E. Golub, F. Wang, J. S McCaskill and Itamar Willner Addressing, amplifying and switching DNAzyme functions by electrochemically-triggered release of metal ions, Chem. Sci., 6, 3544-3549 (2015).
E. Golub, C.-H, Lu and Itamar Willner Metalloporphyrin/G-quadruplexes: From basic properties to practical applications, J. Porphyrins Phthalocyanines, 19, 65-91 (2015).
R. Aizen, E. Golub, A. Trifonov, S. Shimron, A. Niazov‐Elkan and I. Willner G‐Quadruplex‐Stimulated Optical and Electrocatalytic DNA Switches, Small, 11: 3654–3658 (2015).
E. Golub, H. B. Albada, W. C. Liao, Y. Biniuri and I. Willner Nucleoapzymes: hemin/G-quadruplex DNAzyme–aptamer binding site conjugates with superior enzyme-like catalytic functions J. Am. Chem. Soc. 138, 164-172 (2015).
H. B. Albada, E. Golub^† and I. Willner Rational design of supramolecular hemin/G-quadruplex–dopamine aptamer nucleoapzyme systems with superior catalytic performance Chem. Sci. 7, 3092-3101 (2016).
H. B. Albada, J. W. de Vries, Q. Liu, E. Golub, N. Klement, A. Herrmann and I. Willner Supramolecular micelle-based nucleoapzymes for the catalytic oxidation of dopamine to aminochrome Chem. Commun. 52, 5561-5564 (2016).
Y. Biniuri, H. B. Albada, M. Wolff, E. Golub, D. Gelman and I. Willner Cu²⁺ or Fe³⁺ Terpyridine/Aptamer Conjugates: Nucleoapzymes Catalyzing the Oxidation of Dopamine to Aminochrome ACS Catalysis 8, 1802-1809 (2018).
Y. Biniuri, Z. Shpilt, B. Albada, M. Vázquez‐González, M. Wolff, C. Hazan, E. Golub, D. Gelman, and I. Willner A Bis‐Zn2+‐Pyridyl‐Salen‐Type Complex Conjugated to the ATP Aptamer: An ATPase‐Mimicking Nucleoapzyme ChemBioChem 21, 53-58 (2020).
E. Golub, R. H. Subramanian, J. Esselborn, R. G. Alberstein, J. B. Bailey, J. A. Chiong, X. Yan, T. Booth, T. S. Baker, F. A. Tezcan Constructing protein polyhedra via orthogonal chemical interactions Nature, 578, 172-176 (2020).

Research Group

I am currently looking for talented and highly motivated Msc, PhD and postdocs.

Last Updated Date : 29/01/2023